Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in an E. coli expression system.

P01127

Platelet-Derived Growth Factor Beta dimer, PDGF-BB, platelet-derived growth factor beta (homodimer), PDGF-Beta

≥98% by SDS-PAGE and HPLC analysis.

< 1EU per µg of PDGF-BB as determined by the LAL method.

The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is < 1.0 ng/ml.

Lyophilized from a 0.2 µm filtered 0.02M acetic acid solution, pH 6.5.

lyophilized protein

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

≥2 years (lyophilized) at -20°C.

Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.

1. Szabo, A., et al. Stroke 38 (8), 2247-2253 (2007)
2. Guida, T., et al. Clin. Cancer Res. 13 (11), 3363-3369 (2007)

Avoid freeze/thaw cycles.

Cytokine

Recombinant Protein