Mouse Epidermal Growth Factor (GenBank Accession No. NM_010113), a.a. 977- 1029, MW=6.0 kDa, expressed in an E. coli expression system.

Q01279

epidermal growth factor, EGF, beta-urogastrone

≥98% by SDS-PAGE and HPLC analysis.

< 1EU per µg of EGF as determined by the LAL method.

ED50 is 0.1 − 0.2 ng/ml as determined by a cell proliferation assay using BALB/c 3T3 cells.

Lyophilized  from  a  sterile  filtered  solution  containing  10  mM  Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.

lyophilized protein

Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.

≥2 years (lyophilized) at -20°C.

Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.

1. Michaylira, C.Z., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 291 (3), G472-G481
2. Duncan, E.A., et al. J. Biol. Chem. 281 (18), 12521-12525 (2006)

Avoid freeze/thaw cycles.

Cytokine

Recombinant Protein