Human recombinant Flt1 (FMS-like tyrosine kinase), also known as VEGFR1 (Vascular endothelial growth factor receptor 1), GenBank Accession No. AF063657, a.a. 783- end with N-terminal His-tag, MW=67.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Flt1, FMS-like tyrosine kinase 1, VEGFR1, Vascular endothelial growth factor receptor 1

7 pmol/min/μg

Enzyme activity was measure using Z-LYTE assay conditions (Invitrogen). Assay buffer contains 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, 2 μM substrate (Tyr 04, Invitrogen), and Flt1. Reaction was performed at room temperature for 1 hour at 30°C. Developer solution B was added to the reaction and incubated for 1 hour at RT before measuring the fluorescence intensity.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 500 mM imidazole, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Rahimi, N., et al. J. Biol. Chem. 275: 16986-16992, 2000.
2. Shibuya, M. Int. J. Biochem. Cell Biol. 33: 409-420, 2001.

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein