Human PDGFRβ (GenBank Accession No. NM_002609), cytoplasmic domain, a.a. 558-1106(end) with N-terminal His-tag, MW= 62 kDa, expressed in Sf9 cells via a Baculovirus expression system.

P09619

PDGFRβ, PDGFRBeta, Platelet-Derived Growth Factor Receptor, Beta, JTK12, CD140B

15 pmol/min/μg.

The enzyme reaction was carried out for 1 hour at 30°C in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EDTA, 0.01% BRIJ-35 and 200 μM of ATP. Substrate peptide: Tyr2 peptide from Invitrogen.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Claesson-Welsh L. et al. (1988). Mol. Cell. Biol. 8:3476.
2. Heldin C.-H. and Westermark B. (1999). Physiol. Rev. 79:128.
3.Soriano, P. Genes Dev. 1994 Aug 15,8(16):1888-96.
4. Xu, L. et al. Cancer Res. 2005 Jul 1,65(13):5711-9.

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein