Human HER4 kinase, also known as Erb-B4, Proto-Oncogene-Like Protein C-ErbB-4, and Tyrosine Kinase-Type Cell Surface Receptor HER4, GenBank Accession No. NM_005235, a.a. 708-993, with an N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=59.5 kDa.

Q15303

HER4, ERBB4, human epidermal growth factor receptor 4

Aqueous buffer solution.

48 pmol/min/μg.

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)-biotin substrate and 20 mM ATP. Reaction was done at 30°C for 40 min.Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Strunk, K.E. et al., Cancer Res. 67 (14), 6582- 6590 (2007).
2. Law, A.J. et al., Hum. Mol. Genet. 16 (2), 129-141 (2007).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein