Human FGFR2 (GenBank Accession No. NM_000141), a.a. 399-end with N-terminal GST-tag, MW= 74 kDa, expressed in a baculovirus expression system.

FGFR2, Fibroblast Growth Factor Receptor 2, BEK, Keratinocyte Growth Factor Receptor, KGFR, JWS, CD332, FGFR-2

≥150 pmol/min/μg.

Assay was done in Kinase buffer containing 2 mM DTT using Poly-(Glu:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Aqueous buffer solution.

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Holtrich, U. et al., Proc. Nat. Acad. Sci. (1991); 88:10411-10415.

2. Roidl, A. et al., Clin Cancer Res. (2009 Mar 15); 15(6):2058-66.

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein