Human FGFR4 (GenBank Accession No. NM_002011), a.a. 460-end with N-terminal GST-tag, MW= 65 kDa, expressed in Sf9 cells via a baculovirus expression system

FGFR4, Fibroblast Growth Factor Receptor 4, JTK2, CD334, FGFR-4

50 pmol/min/μg

Reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA and 1 mM DTT) containing 20 μM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol and 3 mM DTT

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Holtrich, U. et al., Proc. Nat. Acad. Sci. (1991), 88:10411-10415.
2. Roidl, A. et al., Clin Cancer Res. (2009 Mar 15), 15(6):2058-66.

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein