| 产品名称 | VEGFR2 / NFAT Reporter – HEK293 Recombinant Cell Line |
| 产品货号 | 79387 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 Vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: VEGF (Vascular Endothelial Growth Factor)/VEGFR (Vascular Endothelial Growth Factor Receptor) interaction is an important mediator of angiogenesis as well as cell survival and proliferation. VEGFR2 is a tyrosine receptor kinase that plays a major role in cellular responses driven by VEGF. Targeting the kinase activity of VEGFR2 has been an attractive tool in cancer therapy based on its anti-angiogenesis effects. In addition to the kinase inhibitors, humanized monoclonal antibodies interfering with VEGF/VEGFR2 interaction have been also developed for anti-cancer treatments.
Description: Recombinant HEK-293 cells expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human VEGFR2 (Human Vascular Endothelial Growth Factor Receptor 2; KDR, FLK1; ref seq. NM_002253.1).
Materials Required But Not Provided: • Human VEGF 165 (BPS Bioscience, #91001) Synonym(s): KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1, NFAT
Supplied As: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO.
Host Cell line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
Growth Media: Thaw Medium 1 (BPS Bioscience, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, SV30010.01). Growth Medium 1A (BPS Bioscience, #79528): Thaw Medium 1 (BPS Bioscience, #60187) plus 400 µg/ml of Geneticin (Life Technologies #11811031) and 100 μg/ml of Hygromycin B (Life Technologies, #10687-010). Cells should be grown at 37°C with 5% CO2 using Growth Medium 1A. Assay Medium: DMEM medium (Hyclone #SH30243.01) plus 0.1% BSA. Instructions for use: See data sheet for detailed culturing and assay protocol.
Storage / Stability: Immediately upon receipt, store vials in liquid nitrogen.
Application(s): • Screen for inhibitors of VEGF/VEGFR2 signaling in a cellular context
• Screen for inhibitors of kinase activity of VEGFR2 in a cellular context Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Kinase/Receptor Tyrosine
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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