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产品名称 PDGFRα (D842I) Assay Kit
产品货号 79761
产品价格 现货询价，电话：010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Description:
Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842 mutation is found in gastrointestinal stromal tumors and is associated with resistance to tyrosine kinase inhibitors. The PDGFRα (D842I) Assay Kit is designed to measure PDGFRα (D842I) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Materials Required But Not Provided:

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Synonym(s):
Platelet Derived Growth Factor Receptor Alpha, CD140 Antigen-Like Family Member A, PDGFR2, CD140a Antigen
Supplied As:
The PDGFRα (D842I) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PDGFRα (D842I), Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.
Format:

Catalog #

Reagent

Amount

Storage

100202

PDGFRα (D842I), GST-Tag

10 µg

-80°C

Avoid multiple freeze/ thaw cycles!

79334

5x Kinase Assay Buffer 1

1.5 ml

-20°C

79686

ATP (500 µM)

100 µl

-20°C

40217

Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml)

100 µl

-20°C

79696

96-well plate, white

1

RT

 
Instructions for use:
See assay protocol for detailed instructions.
Storage / Stability:
Up to 6 months when stored as recommended.
Application(s):
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):

1. Evans, E., et al. A Precision Therapy Against Cancers Driven by KIT/PDGFRA Mutations; 2017, Science Translational Medicine Nov; 9(414): 1690.

2. Joglekar-Javadekar, M., et al. Characterization and Targeting of Platelet-Derived Growth Factor Receptor alpha (PDGFRA) in Inflammatory Breast Cancer (IBC). Neoplasia. 2017 Jul; 19(7):564-573.

3. Corless, C.L., et al. PDGFRA mutations in gastrointestinal stromal tumors: frequency, spectrum and in vitro sensitivity to imatinib. J Clin Oncol. 2005 Aug 10; 23(23):5357-64.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Kinase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79761.pdf
产品资料