Human IRAK4 (GenBank Accession No. NM_001114182), a.a. 2-end with N-terminal GST-tag, MW= 77.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

IRAK4, interleukin-1 receptor-associated kinase 4, NY-REN-64, REN64

≥50 pmol/min/µg

Used 0.1 mg/ml MBP as a substrate and 20 μM ATP. Reaction was done for 45 min at 30°C, and the amount of ATP was quantified using Kinase-Glo (Promega)

45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.4 mM KCl, 3 mM DTT, 10% Glycerol and 2 mM Glutathione.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Ku C.L. et al., J. Exp. Med. 204 (10), 2407- 2422 (2007).
2. Hoarau C. et al., J. Immunol. 179 (7), 4754-4765 (2007).

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein