The PD-L2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

activity assay kit, Strep HRP, streptavidin

This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L2, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

1. Lazar-Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Avoid freeze/thaw cycles.

Cell Surface Receptor/Single-pass Transmembrane Protein

Assay Kit