The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit is designed to measure the inhibition of PD-1 binding to PD-L1. The PD-1[Biotinylated]:PD-L1 Homogeneous Assay Kit comes in a convenient AlphaLISA® format with purified biotinylated PD-1, FLAG-tagged PD-L1, and assay buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing biotinylated PD-1 and an inhibitor of choice is incubated with the FLAG-tagged PD-L1 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen

Only limited amounts of DMSO can be included, as it has been shown to disrupt PD-1-PD-L1 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

COMPONENTS:

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for screening for inhibitors of PD-1 binding to PD-L1

1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Avoid freeze/thaw cycles

Immunotherapy

Assay Kit