Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL2[ Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L2 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC, activity assay kit, Strep HRP, streptavidin

This kit comes in a convenient 96-well format, with biotin-labeled PD-L2, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

COMPONENTS:

See assay kit data sheet for detailed protocol.

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

This kit is most useful for screening for inhibitors of PD1 binding to PD-L2.

1. Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Avoid freeze/thaw cycles.

Immunotherapy & Cell Surface Receptors

Assay Kit