世联博研公司现货供应bpsbioscience品牌订货号为72003的PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit - Assay kits

产品名称

PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit - Assay kits - Immunotherapy

产品货号

72003

产品价格

现货询价，电话：010-67529703

产品规格

96 reactions

产品品牌

bpsbioscience

产品概述

产品详情

Background:

Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses.

Description:

The PD-1:PD-L1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-L1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Synonym(s):

activity assay kit, Strep HRP, streptavidin

Supplied As:

Kit comes in a convenient 96-well format, withbiotin-labeled PD-L1, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100binding reactions.

Contraindications:

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Format:

CatalogNumber	Component	Amount	Storage
71105	PD-L1, Biotin-labeled (Human)	5 µg	-80°C	*(Avoid freeze/ thaw cycles!)*
71106	PD-1 (Human)	10 µg	-80°C
	Streptavidin-HRP	15 µl	+4°C
79311	3x Immunobuffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
	HRP chemiluminescent substrate B (brown bottle)	6 ml	+4°C
79699	White 96-well microplate	1	+4°C

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

This kit is useful for screening for inhibitors of PD1 binding to PD-L1

Reference(s):

1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E. et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Cell Surface Receptor/Single-pass Transmembrane Protein

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.

Qty:

Quality Assurance

Product Datasheets

72003.pdf

产品资料