The Mouse PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, mouse PD-L1 is coated on a 96-well plate. Next, mouse PD-1 is incubated with mouse PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), activity assay kit, Strep HRP, streptavidin

This kit comes in a convenient 96-well format, with biotin-labeled mouse PD-1, purified mouse PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

See assay kit data sheet for detailed protocol

One year from date of receipt when stored as directed.

This kit is perfect for screening for inhibitors of mouse PD-L1 binding to mouse PD-1.

1. Lin, D., et al. Proc. Natl. Acad. Sci. USA. 2008, 105: 3011-3016.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.

Avoid freeze/thaw cycles.

Immunotherapy & Cell Surface Receptors

Assay Kit