| 产品名称 | ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit - Assay kits - Immunotherapy |
| 产品货号 | 79673 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 Reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: ICOS (Inducible T-cell Costimulator, CD278) is a costimulatory molecule of the CD28 cell surface receptor superfamily that is expressed on activated T-cells. ICOS is involved in T-cell responses upon binding with its ligand, ICOSL (also known as B7-H2, CD275), which is normally expressed on B-cells, dendritic cells and monocytes. ICOS expression confers an activated phenotype and a strong suppressive capacity to intra-tumoral regulatory T-cells. In particular, the ICOS/ICOSL pathway is required for optimal antitumor responses mediated by anti–CTLA-4 therapy.
Description: The ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of ICOS:ICOSL signaling. The key to this kit is the high sensitivity of detection of biotin-labeled ICOS by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ICOSL is coated on a 96-well plate. Next, ICOS is incubated with ICOSL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Materials Required But Not Provided: PBS (Phosphate buffered saline) Synonym(s): B7 homolog 2, CD275, ICOSL, ICOSLG, B7-related protein (B7RP), B7-H2
Supplied As: This kit comes in a convenient 96- well format, with biotin-labeled ICOS, purified ICOSL, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: Up to 6 months from date of receipt, when stored as recommended.
Application(s): This kit is useful for screening for inhibitors of ICOSL binding to ICOS.
Reference(s): Kieu-Suong, L., et al. Cancer Res. 2016, 76(16): 1–13. Scientific Category: Immunotherapy
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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