Human tryptophan 5- hydroxylase 1 (TPH1) also known as TRPH, GenBank Accession No. NM_004179, a.a. 105-420 with an N-terminal His-tag, MW = 37 kDa, expressed in an E. coli cell expression system.

P17752

tryptophan 5-hyrdoxylase, TRPH

Reaction was performed at room temperature in assay buffer containing 50 mM MOPS, pH 7.0, 60 μM tryptophan, 100 mM ammonium sulfate, 100 μM ferrous ammonium sulfate, 0.5 mM TCEP, 0.3 mM 6-methyl tetrahydropterin, 0.05 mg/ml catalase, 1 mM DTT and TPH1. Fluorescence signal was measured at Em360nm/Ex300nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT and 170 mM imidazole.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Cote, F., et al., Proc. Nat. Acad. Sci. USA. 2003; 100(23): 13525-13530.
2. Walther, D.J., et al., Science. 2003; 299: 76.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Metabolic Enzyme, Immunotherapy

Recombinant Protein