世联博研公司现货供应bpsbioscience品牌订货号为79101-1 79101-2的RANK:RANKL TR-FRET Assay

产品名称

RANK:RANKL TR-FRET Assay - Immunotherapy

产品货号

79101-1 79101-2

产品价格

现货询价，电话：010-67529703

产品规格

96 reactions 384 reactions

产品品牌

bpsbioscience

产品概述

产品详情

Description:

The RANK:RANKL TR-FRET Assay is designed to measure the inhibition of RANK binding to RANKL. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated RANK, RANKL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for one hour. Then, the fluorescence intensity is measured using a fluorescence reader.

Synonym(s):

RANK, RANKL, TNFRSF11A, TNF Receptor Superfamily Member 11a, TNF Superfamily Member 11, TNFSF11, TNF Receptor Superfamily Member 11b, TNFRSF11B, HTRF

Format:

96 Reaction Components

Catalog #	Component	Amount	Storage
70822	RANK, Fc fusion (IgG1), Biotin Labeled (Human)	5 µg	-80°C	Avoid multiple freeze/thaw cycles!
71051	RANKL, His-Tag (Human)	2 µg	-80°C
30017	Anti-His Tb Donor	3 x 10 µl	-20°C
	Dye-labeled Acceptor	3 x 10 µl	-20°C
79311	3x Immuno Buffer 1	4 ml	-20°C
79696	White, 96-well microtiter plate	1 unit	Room. temp

384 Reaction Components

Catalog #	Component	Amount	Storage
70822	RANK, biotinylated	5 µg	-80°C	*(Avoid freeze/thaw cycles!)*
71051	RANKL-His	2 µg	-80°C
30017	Anti-His Tb Donor	2 x 10 µl	-20°C
	Dye-labeled Acceptor	2 x 10 µl	-20°C
79311	3x Immuno Buffer 1	4 ml	-20°C
	White, non-binding, low volume, 384-well microtiter plate	1	Room temp.

Instructions for use:

See assay kit data sheet for detailed protocol

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival

Application(s):

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

1. Annis, A., et al. J. Amer.n Chem. Soc., 2004 May; 126(4): 15495-15503.

2. Yan, T., et al. J. Cell. Biochem. 2001 Aug; 83(2): 320-325.

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

Immunotherapy

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.

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$1,095

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Product Datasheets

79101-1 (96 reactions).pdf 79101-2 (384 reactions).pdf

产品资料