| 产品名称 | PD-1:PD-L2 Cell-Based Inhibitor Screening Assay Kit |
| 产品货号 | 79378 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Programmed Cell Death Protein 1 (PD-1) is a receptor which is expressed on activated T-cells. Binding of PD-1 to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1: PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance.
Description: The PD-1/PD-L2 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L2 interaction. The assay consists of two main components: • Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing Human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment. • Expression vectors for TCR activator and Human PD-L2: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L2 and an engineered cell surface T cell receptor (TCR) activator.
Materials Required But Not Provided: • HEK293 cell and its growth medium (Thaw medium 1, #60187) or other cell lines • Transfection reagent for mammalian cell line [We use Lipofectamine™ 2000 (Life technologies #11668027). However, other transfection reagents work equally well.] • Opti-MEM I Reduced Serum Medium (Life technologies #31985-062) • 96-well tissue culture-treated white clear-bottom assay plate • Luminometer Synonym(s): Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC, activity assay kit, cell based kit, cell-based assay
Supplied As: Each kit contains sufficient reagents for 100 assays using a 96-well plate.
Format: COMPONENTS:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: Store cell line in liquid nitrogen immediately upon receipt. Store other components as described below. Stable for at least 6 months from date of receipt when stored as directed.
Application(s): • Screen for inhibitors of PD-1 signaling in a cellular context • Characterize the biological activity of PD-1 and its interactions with ligands Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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