Human KMO, also known as Kynurenine 3-Monooxygenase, Kynurenine 3-Hydroxylase, and EC 1.14.13.9, Genbank Accession No. NM_003679, a.a. 1-486 (end) with C-terminal His-FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 58 kDa.

O15229

Kynurenine 3-Monooxygenase, Kynurenine 3-Hydroxylase, and EC 1.14.13.9

Aqueous buffer solution.

100 pmol/min/μg

KMO #11307 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 μM NADPH, 400 μM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm were measured using a Tecan Infinite M1000 plate reader.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 μg/ml FLAG peptide, 0.2 mM TCEP and 20% glycerol.

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Zwilling, D., et. al., Cell. 2011; 6(10):863-874.
2. Giorgini, G., et al., Nature Genetics. 2005; 37: 526-531.

Avoid multiple freeze/thaw cycles.

Immunotherapy

Protein