世联博研公司现货供应bpsbioscience品牌订货号为79739-1 79739-2的FGL1:LAG3 TR-FRET Assay Kit

产品名称

FGL1:LAG3 TR-FRET Assay Kit - Immunotherapy

产品货号

79739-1 79739-2

产品价格

现货询价，电话：010-67529703

产品规格

96 reactions 384 reactions

产品品牌

bpsbioscience

产品概述

产品详情

Background:

Lymphocyte-activation gene 3 (LAG3, also CD223) is a cell surface receptor that negatively regulates activation and proliferation of T cells. Fibrinogen-like protein 1 (FGL1), a liver-secreted protein, is a functional LAG3 ligand. Blockade of the FGL1-LAG3 interaction is implicated in promoting antitumor immunity.

Description:

The FGL1:LAG3 TR-FRET Assay is designed to measure the inhibition of LAG3 binding to FGL1 in a homogeneous 96 or 384 reaction format. This TR-FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated LAG3, His-tagged FGL1 protein, and an inhibitor are incubated for one hour. Then, anti-His Tb donor and dye-labeled acceptor are added and fluorescence intensity is measured using a fluorescence reader.

Materials Required But Not Provided:

Fluroescence microplate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer (TR-FRET)

Adjustable micropipettor and sterile tips

Synonym(s):

HFREP1, HP-041, LFIRE-1, LFIRE1, fibrinogen like 1

Format:

96 Reactions:

CatalogNumber	Component	Amount	Storage
100330	FGL1, His tag	5 µg	-80°C	Avoid multiple freeze/thaw cycles!
71147	LAG3 (CD223), Biotin-labeled (Human) HiP™	10 µg	-80°C
30017	Anti-His Tb Donor	2 x 10 µl	-20°C
	Dye-labeled Acceptor	2 x 10 µl	-20°C
	3x FGL1 TR-FRET Buffer	4 ml	-20°C
	White, 96-well microtiter plate	1	Room. temp

384 Reactions:

CatalogNumber	Component	Amount	Storage
100330	FGL1, His tag	2 x 5 µg	-80°C	Avoid multiple freeze/thaw cycles!
71147	LAG3 (CD223), Biotin-labeled (Human) HiP™	2 x 10 µg	-80°C
30017	Anti-His Tb Donor	2 x 10 µl	-20°C
	Dye-labeled Acceptor	2 x 10 µl	-20°C
	3x FGL1 TR-FRET Buffer	4 ml	-20°C
	White, 384-well microtiter plate	1	Room. temp