Woodchuck secreted CTLA4, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152, with N-terminal Avi-TagTM fused to the Fc region of Human IgG1, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Cytotoxic T-lymphocyte-associated protein 4 and CD152

Reaction done similar to human CTLA4:B7-1[biotin] assay kit (BPS Catalog # 72009) protocol. Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% Glycerol

Aqueous buffer solution

At least 6 months at –80°C

1. Dilek, N., et al.,PLOS ONE. 2013 Dec. 23: DOI: 10.1317.

2. Leach, D.R., et al., Science. 1996; 271(5256):1734-1736.

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Immunotherapy

Protein