| 产品名称 | CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit - Immunotherapy |
| 产品货号 | 79695 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 rxns |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: The tumor necrosis factor (TNF) receptor family member CD27 (TNFRSF7) and its TNF-like
ligand CD70 (CD27L, TNFSF7) are a key costimulatory immune checkpoint signal for lymphocyte
activation. Among other effects, this interaction promotes the differentiation of T and B cells and
participates in immunoglobulin synthesis. CD27:CD70 interaction has been demonstrated to have
important roles in autoimmunity, inflammation, infection and cancer, and antibodies targeting
either CD27 or CD70 are in clinical trials for oncology.
Description: The CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit is designed for
screening and profiling inhibitors of CD27:CD70 signaling. This kit comes in a convenient 96- well
format, with biotin-labeled CD70, purified CD27, streptavidin-labeled HRP, and assay buffer for
100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD70
by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay.
First, CD27 is coated on a 96-well plate. Next, CD70 is incubated with CD27 on the plate. Finally,
the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce
chemiluminescence, which can be measured using a chemiluminescence reader.
Materials Required But Not Provided: PBS (Phosphate buffered saline) Synonym(s): S152, S152. LPFS2, CD27LG, TNFSF7
Format:
Instructions for use: See assay protocol.
Storage / Stability: Up to 6 months from date of receipt, when stored as recommended.
Application(s): This kit is useful for screening for inhibitors of CD70 binding to CD27.
Reference(s): Granger, S.W., et al. Cytokine Growth Factor Rev. 2003, 14(3-4): 289-296
Warning(s): Avoid multiple freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
