| 产品名称 | CD19 / Firefly Luciferase - CHO Recombinant Cell Line - Immunotherapy |
| 产品货号 | 79714 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: B-lymphocyte antigen CD19 (Cluster of Differentiation 19), also known as B-Lymphocyte Surface Antigen B4 and CVID3, is a transmembrane protein expressed in follicular dendritic cells and all B lineage cells except plasma cells. CD19 plays two major roles in human B cells. It acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane and it works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. Due to its presence on all B cells, it is a biomarker for B lymphocyte development and lymphoma diagnosis and can be used as a target for leukemia immunotherapies. CD19-targeted therapies based on T cells that express CD19-specific chimeric antigen receptors (CARs) have been utilized for their antitumor abilities in patients with CD19+ lymphoma and leukemia, such as Non-Hodgkins Lymphoma (NHL), CLL and ALL.
Description: Recombinant clonal stable CHO cell line constitutively expressing full length human CD19 protein (also known as B4 or CVID3, Genbank #NM_001770) and the firefly luciferase. Surface expression of CD19 was confirmed by flow cytometry.
Materials Required But Not Provided: • Thaw Medium 3 (BPS Bioscience, #60184) • Growth Medium 3A (BPS Bioscience, #60188) • 96-well tissue culture-treated white clear-bottom assay plate • ONE-Step luciferase assay system (BPS Bioscience, #60690) or other luciferase reagent for measuring firefly luciferase activity • Luminometer Synonym(s): B4, CVID3
Supplied As: Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line: CHO K1 cell line, Chinese Hamster Ovary
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media: Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01). Growth Medium 3A (BPS Bioscience, #60188): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml G418 (Thermo Fisher, #11811031) and 500 μg/ml of Hygromycin B (Thermo Fisher, #10687010) to ensure recombinant expression. Storage / Stability: Store in liquid nitrogen immediately upon receipt.
Application(s): Useful as CD19-expressing target cells in co-culture assay with CD19-CAR-T cells, for both CD19-specific cell killing assay and cytokine production assay.
Reference(s): Application References: 1. Tedder TF, et al. Isolation of cDNAs encoding the CD19 antigen of human and mouse B lymphocytes. A new member of the immunoglobulin superfamily. J. Immunol. 1989 Jul 15;143(2):712 2. Kemeng W, et al. CD19: a biomarker for B cell development, lymphoma diagnosis and therapy. Experimental Hematology & Oncology 2012 1:36 3. Michel S. CD19 CAR T Cells. Cell 2017 Dec 14; 171(7):1471 Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid multiple freeze thaw/cycles
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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