| 产品名称 | c-Mer, GST-tag (Human) |
| 产品货号 | 40254 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 60 kDa
Genbank #: NM_006343
Tag(s): N-terminal GST-tag
a.a: 578–872
Background: c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family (1). The c-MER gene encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain. Functional knockout of c-MER in mice shows the mice to have macrophages deficient in the clearance of apoptotic thymocytes thereby demonstrating the critical role for c-MER in the engulfment and efficient clearance of apoptotic cells. Defects in c-MER have been associated with retinitis pigmentosa, a phenotype that can be reversed by replacement of the c-MER gene product (2).
Description: Human c-Mer, also known as
tyrosine-protein kinase Mer, MERTK, and
receptor tyrosine kinase MerTK GenBank
Accession NM_006343, a.a. 578-872 with N-terminal
GST-tag. Expressed in a
baculovirus-infected Sf9 cell expression
system. MW = 60 kDa.
UniProt Q12866
Synonym(s): tyrosine-protein kinase Mer, MERTK, receptor tyrosine kinase MerTK
Specific Activity: ≥50 pmol/min/µg
Assay Conditions: Assay was done in kinase
buffer containing 1 mM DTT using Poly-
(Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM
ATP. Reaction was done at 30°C for 45 min.
Amount of ATP transferred was calculated
using Kinase-Glo reagent (Promega).
Formulation: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 0.04%
Tween20, 20% glycerol, and 1.6 mM
glutathione
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at –80°C. Application(s): Useful for the study of enzyme kinetics and for inhibitor screening.
Reference(s): 1. Van Hoof, C. et al., Genomics. 1995; 28:
261-272.
2. Chao, Y. et al., Molec. Cell. 2006; 23: 535-546. Warning(s): Avoid freeze/thaw cycles. Storing diluted protein is
not recommended, if necessary, use carrier
protein (BSA 0.1 – 0.5%).
Scientific Category: Kinase/Receptor Tyrosine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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