| 产品名称 | ROR1, GST-tag - CAR T-Cell Therapy |
| 产品货号 | 40396 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 82 kDa
Genbank #: NM_005012
Tag(s): N-terminal GST-tag
a.a: 429–end
Background: ROR1 is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors that play a role in the control of most basic cellular processes including cell proliferation, differentiation, migration and metabolism (1). Many human breast cancers express the ROR1 protein and high-level expression of ROR1 in breast adenocarcinoma is associated with aggressive disease. Silencing expression of ROR1 in human breast cancer cell lines found to express this protein impaired their growth in vitro and also in immune-deficient mice (2).
Description: Recombinant human ROR1 (GenBank Accession No. NM_005012), a.a. 429-end, with N-terminal GST-tag, MW 82 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s): dJ537F10.1; NTRKR1, neurotrophic tyrosine kinase, receptor-related 1, ROR
Purity: ≥95%
Specific Activity: 0.2 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM beta-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate ROR1 with 0.25 mg/ml Myelin Basic
Protein substrate and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Afzal, A. R. et.al. Hum. Mutat. 22: 1-11, 2003.
2. Zhang, S. et al. PLoS One. 2012;7(3):e31127. 3. Channavajhala, P., et al., J. Biol. Chem. 2003,278:47089-47097. 4. Rodriguez, C., et al., Cell Signal 2006,18(9):1376-1385. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Receptor Tyrosine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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