Human secreted CD58-Fc fusion protein, also known as LFA-3, GenBank Accession No. NM_001779, a.a. 29-215, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

0.87 mg/ml

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.

1. Wang, J., et al., Cell. 1999 Jun; 97(6):791-803.

2. Raychaudhuri, S., et al., Nature. 2009 Nov ;41:1313-1318.

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Immunotherapy

Protein