Three types of arginine methylation exist in mammalian cells: monomethylarginine (MMA), asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA). Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze ADMA, while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. Type II PRMTs are found to be strongly implicated in diseases like cancer. For example, PRMT5 plays a role in the repression of certain tumor suppressor genes such as RB tumor suppressors while PRMT7 overexpression is observed in breast cancer
| 产品名称 | PRMT Protein, Biotin |
| 产品货号 | 52102 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: HEK293
MW: 73 kDa (PRMT5), 38 kDa (MEP50)
Genbank #: NM_006109 (PRMT5), NM_024102 (MEP50)
Tag(s): N-terminal FLAG-Avi-tag (PRMT5), N-terminal His-tag (MEP50)
a.a: 2–end (both)
Background: Description: PRMT5/MEP50 complex of human PRMT5 (GenBank Accession No. NM_006109), amino-acids 2-637(end), with N-terminal FLAG-Avi tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102) with N-terminal His-Tag, amino-acids 2-342(end), MW=38 kDa, coexpressed in HEK293 cells and enzymatically biotin-labeled using Avi-tag™ technology.
Synonym(s): PRMT5, HRMT1L5, IBP72, JBP1, SKB1, PRMT Protein, MEP50
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/mL FLAG peptide, 20% glycerol and 3 mM DTT.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Reference(s): 1. Zhao Q. et al., J. Nat. Struct. Mol. Biol. 16 (3), 304-311 (2009). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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