| 产品名称 | PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit - Biotinylation |
| 产品货号 | 72026 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: There have been a number of studies defining a role for PD-L1 in the
regulation of immune tolerance and cancer immunity through binding to T cell-expressed PD-1.
Interestingly, PD-L1 has also been shown to interact with B7-1, also known as CD80. Like PD-
1:PD-L1 interaction, B7-1:PD-L1 signaling also leads to inhibition of T cell activation,
proliferation, and cytokine production, suggesting that this pathway may also serve as a target
in cancer immunotherapy and inflammatory diseases.
Description: The PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for
screening and profiling inhibitors of the PD-L1:B7-1 interaction. The key to this kit is the high sensitivity of detection of
biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are
required for the assay. First, PD-L1 is coated on a 96-well plate. Next, B7-1-biotin is incubated
with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition
of an HRP substrate to produce chemiluminescence, which can then be measured using a
chemiluminescence reader.
Synonym(s): Programmed Cell Death 1 Ligand 1, CD274, B7 homolog 1 (B7-H1), CD80, CTLA-4 Counter-Receptor B7.1 , activity assay kit, Strep HRP, streptavidin
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-1 (CD80), purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format:
Instructions for use: See assay kit data sheet for detailed protocol
Storage / Stability: Stable for one year from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s): This kit is useful for screening for inhibitors of PD-L1 binding to B7-1.
Reference(s): 1. Butte, M.J., et al., Immunity. 2007; 27(1): 111-122.
2. Butte, M.J. et al., Mol. Immunol. 2008; 45: 3567-3572. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Immunotherapy
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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| 产品资料 |
