| 产品名称 | Histone Octamer, Biotin-labeled, His-tag - Other Biotinylated Products - Biotinylation |
| 产品货号 | 52061 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 50 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 117 kDa
Genbank #: NM_033445, NM_003528, NM_003532, and NM_003548
Tag(s): N-terminal His-tag, Biotin
Background: The histone octamer is composed of a central heterotetramer of two copies of both histones H3 and H4, flanked by two heterodimers of histones H2A and H2B. The octamer assembles when the H3/H4 tetramer complexes with two H2A/H2B dimers. These histones of the histone octamer all contain N-terminal tails that emanate from their central histone folds and are targets for epigenetic modification.
Description: Human recombinant histone octamer consisting of 2 molecules each of N-terminal His-tagged histones H2A (a.a. 2-130(end)), H2B (a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. H2B has been biotin-labeled via thiol modification of an additional C-terminal cysteine prior to assembly. Global MW = 117 kDa.
UniProt Q7L7L0, Q16778, P68431, P62805
Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol.
Storage / Stability: >6 months at –80°C Application(s): Substrate for histone methyltransferase or acetyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases and acetyltransferases for drug discovery and HTS applications.
Reference(s): 1. Miller KM, Jackson SP. Biochem Soc Trans 40(2):370-6 (2012). Notes: Since the recombinant histone proteins in this octamer were prepared in E. coli, they will not have methyl, acetyl or many other epigenetic marks.
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Substrate
Data shown is lot-specific. Contact us for specific information on other lots.
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