Histone H3 peptide (a.a. 1-21), di-methylated at Lysine 9 with a C-terminal GG linker followed by a biotinylated Lys at its C-terminus.

H3K9me2, Histone H3 Lys9me2

≥95% by HPLC

40 µM in water (500 µl)

Aqueous buffer solution

≥ 6 months at -20°C or -70°C

Suitable as a substrate for histone demethylases belonging to the Jumonji (JMJ) family. It can be used to study enzyme kinetics, and to screen small molecular inhibitors of these demethylases for drug discovery and HTS applications.

1. Grewal, S., et al., Curr Opin Cell Biol. 2004;16:230.
2. Rice, J., et al., Mol Cell. 2003;12: 1591.

Avoid freeze/thaw cycles.

ARTKQTAR-K(Me2)-STGGKAPRKQLA-GGK(Biotin)

Methyltransferase

Substrate