Biotin ligase BirA, GenBank Accession No. AP012306, a.a. 2 - 321 (end) with N-terminal His-FLAG tag, MW = 37 kDa, expressed in a baculovirus infected Sf9 cell expression system.

H0QFJ5

Biotin ligase BirA

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 80 mM imidazole.

Aqueous buffer solution

At least 6 months at -80°C

Useful for covalent in vitro biotin-labeling of receptor proteins for detection and purification assays.

1. Li, Y., et al., Protein Expr Purif. 2012 Mar; 82(1): 162-7.

2. Chakravartty, V., et al., J Bacteriol. 2012 Mar; 194(5): 1113-26.

Avoid freeze/thaw cycles.

Miscellaneous

Recombinant Protein