Woodchuck B7-1, also known as CD80, with C-terminal Avi-Tag fused to the Fc-region of Human IgG1. a.a 30-237, expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 52 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

CD80, with C-terminal Avi-Tag fused to the Fc-region of Human IgG1

Reaction done similar to human CTLA4:B7-1[biotin] assay kit (BPS Catalog # 72009) protocol. Buffer is PBS with 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. The coat protein, woodchuck CTLA4 (wCTLA4), was added ON at 4 deg (various concentrations as indicated). Binding reaction initiated with addition of woodchuck B7-1-biotin (wB7-1-biotin) for 2h at RT. Binding was detected using Strep-HRP generated luminescence.

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

At least 6 months at –80°C

1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081.

2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Immunotherapy

Protein