Human x-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4, a.a. 120 – 356 containing Bir2 and Bir3 domains (GenBank Accession No. NM_001167) with N-terminal His-tag, MW = 28.3 kDa, expressed in an E. coli expression system.

P98170

XIAP, IAP-like protein, API3, X-linked IAP, BIRC4

50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at exc 475 nm, em 528 nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.

Aqueous buffer solution

>6 months at -80°C.

Useful in binding and inhibition studies of Caspase 9, screening inhibitors, and selectivity profiling.

1. Nikolovska-Coleska, Z., et al., Anal Biochem. 2008 Mar 1;374(1):87-98.
2. Verhagen, A.M., et al., Cell Death Differ. 2007 Feb;14(2):348-57
3. Fulda, S., Vucic., D., Nat Rev Drug Discov. 2012 Feb 1;11(2):109-24

Avoid freeze/thaw cycles

Apoptosis

Recombinant Protein