The MCL-1 TR-FRET Assay Kit is designed to measure the inhibition of MCL-1 binding to its ligand in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, MCL-1, peptide ligand, and an inhibitor is incubated for 3 hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Dithiothreitol (DTT, 0.5 M)

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)

Adjustable micropipettor and sterile tips

mcl1, HTRF

See assay kit data sheet for detailed protocol

At least 6 months from date of receipt when stored as directed.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

1. Abid, M., et al. Curr Med Chem 2017; 24:4488.

Avoid freeze/thaw cycles

Apoptosis

Assay Kit