Mouse MALT1 also known as IMD12, MLT, MLT1, PCASP1, and MALT1 paracaspase, GenBank Accession No. NM_172833, a.a. 2-832 (end), and expressed in a Baculovirus infected Sf9 cell expression system. MW = 93 kDa.

IMD12, MLT, MLT1, PCASP1, MALT1 paracaspase

The assay was performed by fluorescence using a recombinant mMALT1 and Ac-LRSR-AMC as substrate. The fluorescence at (ex 365 em 460 nm) is correlated with the amount of product formed.All of the reactions were conducted at 30 ºC. The reaction mixture is in either 96 well black nunc plates or 384 well black Greiner plates as indicated. The reaction in MALT1 Assay Buffer (50 mM MES, 150 mM NaCl, 0.1% CHAPS, 1 M Na.citrate, 10 mM DTT added fresh, pH 6.8) contains 0-500 µM Ac-LRSR-AMC, and 0- 2 µg MALT1. The reaction mixture incubated for up to 3h while reading the fluorescence signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals were measured using a Tecan Infinite M1000 plate reader.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol 100ng/ul flag peptide

At least 6 months at –80°C.

Useful as a positive control for western blot studies.

1. Lijun, S., et al., Molecular Cell. 2004. May 14;3: 289-301.

2. Lucas, P., et al., JBC. 2001. June 276: 19012-19019.

Avoid freeze/thaw cycles.

Proteases

Protein