Recombinant HEK293 cell line expressing a full length human CD137 (NM_003811). The NF-ĸB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by human CD137 ligand, NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

CD137, 4-1BB

HEK293

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination.

Thaw Medium 1 (BPS Bioscience, #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Thermo Fisher, Cat. #26140079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1G (BPS Bioscience, #79544): Thaw Medium 1 (BPS Bioscience, #60187) plus 400 µg/ml G418 (Geneticin) and 50 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.

Store in liquid nitrogen immediately upon receipt.

Screen for activators or inhibitors of CD137 signaling in a cellular context and characterize the biological activity of CD137 and its interactions with ligands

1. McNamara II JO et.al. (2008) Multivalent 4-1BB binding aptamers co-stimulate CD8+ T cells and inhibit tumor growth in mice. J. Clin. Invest. 118: 376-386

2. Ma BY et.al. (2005) The expression and regulatory role of OX40 and 4-1BB heterodimers in activated human T cells. Blood. 106: 2002-2010. 

Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Immunotherapy

Cell Line