| 产品名称 | BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit |
| 产品货号 | 79667 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: The TNF family ligand B-cell Activating Factor (BAFF, also known as BLyS, TALL-1 or CD257, encoded by the TNFSF13B gene) is a Type II membrane-bound protein, which can be released as a soluble ligand upon proteolytic processing. This cytokine is a ligand for the receptors Transmembrane Activator and CAML Interactor (TACI, TNFRSF13B), BAFF Receptor (BAFF-R, TNFRSF13C) and B-cell Maturation Antigen (BCMA, TNFRSF17). These interactions promote cell survival and they play a crucial role in B cell development. In particular, BCMA signaling is an important player in the later stages of B cell differentiation, including the survival of long-lived bone marrow plasma cells and likely for the survival of plasmablasts.
Description: The BAFF:BCMA[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of BAFF:BCMA signaling.The key to this kit is the high sensitivity of detection of biotin-labeled BCMA by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, BAFF is coated on a 96-well plate. Next, BCMA is incubated with BAFF on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Materials Required But Not Provided: PBS (Phosphate buffered saline) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Adjustable micropipettor and sterile tips Synonym(s): BAFF, BLYS, TALL-1, CD257, TNFRSF13C, B-cell activating factor, TNF13b
Supplied As: This kit comes in a convenient 96- well format, with biotin-labeled BCMA, purified BAFF, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Format:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: Up to 6 months from date of receipt, when stored as recommended.
Application(s): This kit is useful for screening for inhibitors of BCMA binding to BAFF.
Reference(s): Thompson, J. S., et al. J. Exp. Med. 2000, 192(1): 129-136 Bossen, C., et al. Semin. Immunol. 2006, 18(5): 263-275 Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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