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产品名称 PRMT5 TR-FRET Assay Kit
产品货号 52171
产品价格 现货询价，电话：010-67529703
产品规格 384 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Background:

PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. PRMT5 is a critical regulatory-protein linked to genome organization, cell cycle regulation, and stem cell differentiation. PRMT5 transfers methyl groups from S-adenosylmethoinine (SAM) to form mono and symmetric di-methylated arginine residues in histone proteins, transcription factors, and other regulatory proteins. Given its regulatory function, PRMT5 is believed to contribute to tumorigenesis. Its dysregulated activity has been associated with many cancers including lymphomas, lung, and breast cancer. Additionally, PRMT5 has been linked to other diseases including kidney and heart disease as well as neurological disorders such as Huntington’s and Alzheimer’s diseases.

Description:
The PRMT5 TR-FRET Assay Kit is designed to measure PRMT5 activity in a homogeneous 384 reaction format. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The key to the PRMT5 TR-FRET Assay Kit is a highly specific antibody that recognizes methylated substrate.
Synonym(s):
PRMT5, HRMT1L5, IBP72, JBP1, SKB1, HTRF
Supplied As:
The PRMT5 TR-FRET Assay Kit comes in a convenient format, with histone H4 peptide substrate, a Eu-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, FRET detection buffer, dye-labeled acceptor, and purified PRMT5.
Format:

COMPONENTS:

Catalog #
 
Component
 
Amount
 
Storage
 
51048
 
PRMT5/MEP50
 
40 µg
 
-80°C
 
Avoid freeze/ thaw cycles!
 
52120
 
60 µM S-adenosylmethionine
 
250 µl
 
-80°C
 
 
 
Eu-labeled antibody
 
5 µl
 
-80°C
 
 

Biotinylated histone H4 peptide substrate

1000 rxns

-80°C

 
 

4x PRMT5 assay buffer*

3 x 1 ml

 
-20°C

 

Dye-labeled acceptor

2 x 10 µl

-20°C

 

PRMT5 TR-FRET Detection Buffer

4 ml

-20°C

White, nonbinding, low volume, microtiter plate

1

Room temp.

 
* Add 10 µl of 0.5 M DTT per vial before use.
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):
Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

52171.pdf
产品资料