世联博研公司现货供应bpsbioscience品牌订货号为79684的LIGHT:HVEM [Biotinylated] Inhibitor Screening Assay Kit产品

产品名称

LIGHT:HVEM [Biotinylated] Inhibitor Screening Assay Kit

产品货号

79684

产品价格

现货询价，电话：010-67529703

产品规格

96 Reactions

产品品牌

bpsbioscience

产品概述

产品详情

Background:

Herpesvirus entry mediator (HVEM, also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14) or CD270) and LIGHT (tumor necrosis factor superfamily member 14 (TNFSF14) or CD258) mediate costimulatory responses in T cell activation and proliferation during the immune response. The HVEM/LIGHT co-signaling pathway plays important roles in several biological processes, including autoimmunity, inflammation, bacterial and virus infection, transplant rejection and cancer.

Description:

The LIGHT:HVEM[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT:HVEM signaling. The key to this kit is the high sensitivity of detection of biotin-labeled HVEM by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LIGHT is coated on a 96-well plate. Next, HVEM is incubated with LIGHT on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Materials Required But Not Provided:

PBS (Phosphate buffered saline)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Synonym(s):

TNFSF14, CD258, HVEML, LIGHT, LTg, TR2, TNLG1D, tumor necrosis factor superfamily member 14, TNF superfamily member 14

Format:

Catalog #	Component	Amount	Storage
71266	LIGHT, His-Tag (Human)	10 µg	-80 °C	Avoid multiple freeze/thaw cycles!
71143	HVEM, Fc fusion, Biotin-labeled (Human)	5 µg	-80 °C
79311	3x Immuno Buffer 1	50 ml	-20 °C
79728	Blocking Buffer 2	50 ml	+4 °C
	Streptavidin-HRP	15 µl	-20 °C
	HRP chemiluminescent substrate A (transparent bottle)	6 ml	+4 °C
	HRP chemiluminescent substrate B (transparent bottle)	6 ml	+4 °C
79699	96-well white microplate	1	+4 °C

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

This kit is useful for screening for inhibitors of HVEM binding to LIGHT.

Reference(s):

Granger, S.W., et al. Cytokine Growth Factor Rev. 2003, 14(3-4): 289-296
Del Rio, M.L., et al. J. Leukoc. Biol. 2010, 87(2): 223-235

Warning(s):

Avoid multiple freeze/thaw cycles.

Scientific Category:

Immunotherapy

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.

Qty:

Quality Assurance

Product Datasheets

79684.pdf

产品资料