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产品名称 EZH2 Chemiluminescent Assay Kit
产品货号 52009L
产品价格 现货询价，电话：010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Background:
EZH2 is a histone methyltransferase that exhibits methylation activity toward H3-K27.
Description:
The EZH2 Direct Activity Chemiluminescent Assay Kit is designed to measure activity of the EZH2 complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling applications. The key to the EZH2 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH
Supplied As:
Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, reagents, and purified EZH2 complex for 100 enzyme reactions.
Contraindications:
DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:

Catalog #

Component

Amount

Storage

51004

EZH2/EED/SUZ12/RbAp48/AEBP2

25 µg

-80°C

(Avoid freeze/ thaw cycles!)

52120

400 µM S-adenosylmethionine

250 µl

-80°C

52140F

Primary antibody 6

12.5 µl

-80°C

52131H

Secondary HRP-labeled antibody 2

10 µl

-80°C

52170

4x HMT assay buffer 2

3 ml

-20°C

52100

Blocking buffer 4

50 ml

+4°C

 

HRP chemiluminescent substrate  

(2 components)

6 ml each

+4°C

 

96-well plate precoated with histone substrate

1 plate

+4°C
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Dillon S.C., et al. 2005. Genome Biology 6:227.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

52009L.pdf
产品资料