Human VEGFR3 also known as Flt-4, Fms-Related Tyrosine Kinase 4, FLT4, and Vascular Endothelial Growth Factor Receptor 3, GenBank Accession No. NM_182925, a.a. 798-1298 with Nterminal GST-tag, expressed in baculovirus expression system. MW= 82 kDa.

P35916

Flt-4, Fms-Related Tyrosine Kinase 4, FLT4, VEGFR3, Vascular Endothelial Growth Factor Receptor 3

Aqueous buffer solution.

20 pmol/min/μg.

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase- Glo reagent from Promega.

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Goodyear S.M. et al., Prostate 69 (9), 982-990 (2009).
2. Ghalamkarpour A. et al., J. Med. Genet. 46 (6), 399-404 (2009).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein