Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), a.a. 1–2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system.

Q13085

acetyl-Coenzyme A carboxylase alpha transcript variant 1, ACC-1, ACC 1, ACC -1 acetyl-coa carboxylase acetyl coa acetyl coenzyme a

≥58 pmol/min/µg.

A 25 μl ACC1 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl₂, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO₃ and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected using ADP-Glo® Luminescent Assay Platform (Promega).

50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol, 1 mM DTT, 100 µg/ml FLAG peptide

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sinilnikova, O.M. et al., Cancer Epidemiol. Biomarkers Prev. 16 (3), 409-415 (2007)
2. Olsen, J.V. et al., Cell 127 (3), 635-648 (2006)

Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)

Avoid freeze/thaw cycles.

Metabolic Enzymes-Carboxylase

Recombinant Protein