The EZH2-EED Binding Assay Kit is designed to measure the inhibition of the protein-protein interaction between EZH2 and EED. The key to this kit is the specific binding of EZH2 to EED. With this kit, only two simple steps on a microtiter plate are required. First, incubate a sample containing EZH2, EED and an inhibitor of choice. Next, incubate with a mixture of acceptor and donor beads, followed by reading the Alpha-counts.

KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH, EED

The kit comes in a convenient AlphaLISA® format, with GST-tagged EZH2, FLAG-tagged EED, and enough assay buffer to perform a total of 384 reactions.

Assay was done using AlphaLISA format to measure the inhibition of the protein-protein interaction between EZH2 and EED. Reaction was performed at room temperature for 1 hour. Anti-FLAG Acceptor Beads and Glutathione Donor Beads were added and the reaction was incubated for 1 hour at room temperature. Alpha Counts were read on AlphaScreen microplate reader.

See product datasheet for detailed assay protocol.

At least one year from date of receipt when stored as directed.

Useful for the study of EZH2 binding assays, screening inhibitors and selectivity profiling.

Kong, X., et al., J. Med. Chem. 2014; 57:9512.

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Anti-FLAG AlphaLISA® Acceptor Beads Beads, 5 mg/ml (PerkinElmer #AL112C)
AlphaScreen® Glutathione Donor Beads, 5 mg/ml (PerkinElmer #6765300)
Optiplate-384 (PerkinElmer #6007290) AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit