Human DNMT1 (GenBank Accession No. NM_001130823), a.a. 501-1632 (end), with N-terminal His-FLAG-tags, MW=130 kDa, expressed in Sf9 cells via a baculovirus expression system.

P26358

TRDMT1, DNA, DNA (cytosine-5)-methyltransferase 1, DNMT1, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNA (cytosine-5-)-methyltransferase 1

≥0.16 pmol/min/µg.

Assay was performed using the DNMT1 Assay kit, BPS Cat. #52050L, using 40 µM S-adenosylmethionine for 1 hour at 37°C.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tan, H.H. and Porter, A.G. Biochem. Biophys. Res. Commun. 382 (2), 462-467 (2009).
2. Fan, H. et al., World J. Gastroenterol. 15 (16), 2020-2026 (2009).

Application Reference(s):
1. Electrical Probes of DNA-Binding Proteins. (2017)
2. Antroquinonol D, isolated from Antrodia camphorata, with DNA demethylation and anticancer potential (2014)
3. DNA 5-methylcytosine demethylation activities of the mammalian DNA methyltransferases (2013)
4. DNMT1-interacting RNAs block gene-specific DNA methylation (2013)
5. Electrochemical assay for the signal-on detection of human DNA methyltransferase activity (2013)
6. Methylation of RASSF1A gene promoter is regulated by p53 and DAXX (2013)

Avoid freeze/thaw cycles.

Methyltransferase

Recombinant Protein