Human EGFR T790M, C797S, L858R (GenBank Accession No. NM_005228), (a.a. 668-1210) with C-terminal His-Tag and N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.

P00533

EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA

≥150pmol/min/μg

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol

Aqueous buffer solution

At least 6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Thress, K.S., et al., Nat. Med. 2015; 21:560-562.
2. Niederst, M.J., et al., Clin. Canc. Res. 2015.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase

Recombinant Protein