世联博研公司现货供应bpsbioscience品牌订货号为79380 60690-1 Size: 10 ml $125 Catalog #: 60690-2 Size: 100 ml $875 Catalog #: 60690-5 Size: 500 mL $3,450 Catalog #: 60690-3 Size: 1 L $6,595 Background: Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation. One Step Luciferase Assay System The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer. Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive – highly sensitive detection of firefly luciferase activity. • Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient – simple one-step, homogeneous protocol. • High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation – does not require a luminometer with injectors. Synonym(s): Luciferin, Firefly luciferase, luciferase buffer, one step, step, luciferase detection buffer Format: Components for 10 ml Size See datasheet for further information. Instructions for use: See product datasheet for detailed protocol. Storage / Stability: At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible. Application(s): • Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. • High-throughput screening using luciferase reporter cell lines Notes: • The reagent has been validated in a 96-well format. Other formats will require scaling and optimization by the end-user. • Luciferase Reagent Buffer must be at ~ room temperature (20- 25°C) before use. • Luciferase Assay Reagent should be added to cells for at least 5 minutes before measuring luminescence to allow complete cell lysis. • For maximal light intensity, measure samples within 1 hour of reagent addition. • Avoid exposing to excessive heat or light during incubation. • Different cell lines may exhibit variation in lysis ability and/or luminescence signal and may require slight optimization by the end-user. • Luminescence signal is affected by assay conditions. Results should be compared between samples measured using the same cell type and media/serum combinations. • To analyze multiple plates, include a common control sample in each plate and normalize the luminescence of each plate to the control contained in the same plate. • Background luminescence is a characteristic of luminometer performance, therefore, background luminescence must be subtracted from all readings for accuracy. Warning(s): Protect from light. Avoid freeze/thaw cycles. Scientific Category: Cell-Based Assay Kits Product Type: Reagent Data shown is lot-specific. Contact us for specific information on other lots. *Size * Required Fields Qty: 1 ADD TO CART Bulk QuoteContact Us Quality Assurance Product Datasheets 60690-1.pdf 60690-2.pdf 60690-5.pdf 60690-3.pdf Related Products NFAT Reporter (Luc) – Jurkat Recombinant Cell Line ABL (T315I) Assay Kit TIGIT / NFAT Reporter - Jurkat Cell Line PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line BTLA / NFAT - Luciferase Reporter - Jurkat Recombinant Cell Line" target="_blank">ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)• Luminometer的CSF1R / SRE – Reporter HEK293 Recombinant Cell Line

产品名称	CSF1R / SRE – Reporter HEK293 Recombinant Cell Line - Cell Line Rentals
产品货号	79380 60690-1 Size: 10 ml $125 Catalog #: 60690-2 Size: 100 ml $875 Catalog #: 60690-5 Size: 500 mL $3,450 Catalog #: 60690-3 Size: 1 L $6,595 Background: Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation. One Step Luciferase Assay System The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer. Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive – highly sensitive detection of firefly luciferase activity. • Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient – simple one-step, homogeneous protocol. • High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation – does not require a luminometer with injectors. Synonym(s): Luciferin, Firefly luciferase, luciferase buffer, one step, step, luciferase detection buffer Format: Components for 10 ml Size See datasheet for further information. Instructions for use: See product datasheet for detailed protocol. Storage / Stability: At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible. Application(s): • Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. • High-throughput screening using luciferase reporter cell lines Notes: • The reagent has been validated in a 96-well format. Other formats will require scaling and optimization by the end-user. • Luciferase Reagent Buffer must be at ~ room temperature (20- 25°C) before use. • Luciferase Assay Reagent should be added to cells for at least 5 minutes before measuring luminescence to allow complete cell lysis. • For maximal light intensity, measure samples within 1 hour of reagent addition. • Avoid exposing to excessive heat or light during incubation. • Different cell lines may exhibit variation in lysis ability and/or luminescence signal and may require slight optimization by the end-user. • Luminescence signal is affected by assay conditions. Results should be compared between samples measured using the same cell type and media/serum combinations. • To analyze multiple plates, include a common control sample in each plate and normalize the luminescence of each plate to the control contained in the same plate. • Background luminescence is a characteristic of luminometer performance, therefore, background luminescence must be subtracted from all readings for accuracy. Warning(s): Protect from light. Avoid freeze/thaw cycles. Scientific Category: Cell-Based Assay Kits Product Type: Reagent Data shown is lot-specific. Contact us for specific information on other lots. Size Required Fields Qty: 1 ADD TO CART Bulk QuoteContact Us Quality Assurance Product Datasheets 60690-1.pdf 60690-2.pdf 60690-5.pdf 60690-3.pdf Related Products NFAT Reporter (Luc) – Jurkat Recombinant Cell Line ABL (T315I) Assay Kit TIGIT / NFAT Reporter - Jurkat Cell Line PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line BTLA / NFAT - Luciferase Reporter - Jurkat Recombinant Cell Line" target="_blank">ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)• Luminometer
产品价格	现货询价，电话：010-67529703
产品规格	2 Vials 10 ml $125 Catalog #: 60690-2 Size: 100 ml $875 Catalog #: 60690-5 Size: 500 mL $3,450 Catalog #: 60690-3 Size: 1 L $6,595 Background: Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation. One Step Luciferase Assay System The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer. Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive – highly sensitive detection of firefly luciferase activity. • Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient – simple one-step, homogeneous protocol. • High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation – does not require a luminometer with injectors. Synonym(s): Luciferin, Firefly luciferase, luciferase buffer, one step, step, luciferase detection buffer Format: Components for 10 ml Size See datasheet for further information. Instructions for use: See product datasheet for detailed protocol. Storage / Stability: At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible. Application(s): • Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. • High-throughput screening using luciferase reporter cell lines Notes: • The reagent has been validated in a 96-well format. Other formats will require scaling and optimization by the end-user. • Luciferase Reagent Buffer must be at ~ room temperature (20- 25°C) before use. • Luciferase Assay Reagent should be added to cells for at least 5 minutes before measuring luminescence to allow complete cell lysis. • For maximal light intensity, measure samples within 1 hour of reagent addition. • Avoid exposing to excessive heat or light during incubation. • Different cell lines may exhibit variation in lysis ability and/or luminescence signal and may require slight optimization by the end-user. • Luminescence signal is affected by assay conditions. Results should be compared between samples measured using the same cell type and media/serum combinations. • To analyze multiple plates, include a common control sample in each plate and normalize the luminescence of each plate to the control contained in the same plate. • Background luminescence is a characteristic of luminometer performance, therefore, background luminescence must be subtracted from all readings for accuracy. Warning(s): Protect from light. Avoid freeze/thaw cycles. Scientific Category: Cell-Based Assay Kits Product Type: Reagent Data shown is lot-specific. Contact us for specific information on other lots. Size Required Fields Qty: 1 ADD TO CART Bulk QuoteContact Us Quality Assurance Product Datasheets 60690-1.pdf 60690-2.pdf 60690-5.pdf 60690-3.pdf Related Products NFAT Reporter (Luc) – Jurkat Recombinant Cell Line ABL (T315I) Assay Kit TIGIT / NFAT Reporter - Jurkat Cell Line PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line BTLA / NFAT - Luciferase Reporter - Jurkat Recombinant Cell Line" target="_blank">ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)• Luminometer
产品品牌	bpsbioscience
产品概述
产品详情	Background: Colony Stimulating Factor 1 Receptor (CSF1R, CSFR, CD115, M-CSF-R) is a single-pass tyrosine kinase transmembrane receptor which is part of the type III protein tyrosine kinase receptor family. CSF1R is activated by either of two cytokines, CSF1 (MCSF; CSF-1) and IL-34 (IL34), causing homodimerization and activation of downstream kinase activity. CSF1R is expressed on the surface of monocytes and macrophages, and its activation controls the growth, function, and differentiation of macrophages. This interaction is used by numerous cancer types, such as diffuse-type tenosynovial giant cell tumors (dt-GCT), to evade the immune system. By overexpressing the cytokine CSF1, these cells drive the development and survival of Tumor-Associated Macrophages (TAMs), which in turn suppress the local immune response to the cancer. Activation of CSF1R by its ligand initiates a vast array of intracellular activity, including activation of the MAPK/ERK signaling pathway. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. Description: The CSF1R SRE HEK293 recombinant cell line has been transfected with full-length human CSF1R cDNA (NP_005202) under a CMV promoter for high constitutive expression. The SRE–luciferase reporter is also stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of the Serum Response Element upstream of a minimal promoter. Upon ligand binding, active CSF1R will initiate the MAPK/ERK signaling pathway, leading to expression of the SRE-controlled luciferase reporter. Materials Required But Not Provided: • Thaw Medium 1 (BPS Bioscience, #60187) • Growth Medium 1M (BPS Bioscience, #79723) • Assay Medium 1B (BPS Bioscience, #79617): MEM medium (Hyclone, #SH30024.01) supplemented with 0.5% FBS (Thermo Fisher, #26140079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01). • Recombinant human CSF1 (BPS Bioscience, #90215) • 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate • ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690) • Luminometer Synonym(s): CSF1R, CSFR, CD115, M-CSF-R Supplied As: Two vials containing ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS. Host Cell line: HEK293 Growth Media: Thaw Medium 1 (BPS Bioscience, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Thermo Fisher, #26140079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, SV30010.01). Growth Medium 1M (BPS Bioscience, #79723): Thaw Medium 1, 400 μg/ml of Geneticin (Thermo Fisher, #11811031) and 0.5 μg/ml Puromycin Dihydrochloride (Thermo Fisher, #A1113803). Storage / Stability: Store in liquid nitrogen immediately upon receipt. Do not store for long-term at -80°C or on dry ice. Application(s): Monitor the CSF1R/MAPK/ERK signaling pathway activity and SRF-mediated activity. Screen for activators or inhibitors of the CSF1R/MAPK/ERK signaling pathway. Reference(s): 1. Cannarile, Michael A., et al. 2017. “Colony-Stimulating Factor 1 Receptor (CSF1R) Inhibitors in Cancer Therapy.” Journal for Immunotherapy of Cancer, 5(1),(53), doi:10.1186/s40425-017-0257-y. 2. Liu, Yang, and Xuetao Cao. 2014. “The Origin and Function of Tumor-Associated Macrophages.” Cellular and Molecular Immunology, 12(1), 1–4., doi:10.1038/cmi.2014.83. 3. Luo, Jian, et al. 2013. “Colony-Stimulating Factor 1 Receptor (CSF1R) Signaling in Injured Neurons Facilitates Protection and Survival.” The Journal of Experimental Medicine, 210(1), 157–172., doi:10.1084/jem.20120412. 4. Yao, G.-Q., et al. 2005. “CSF-1 Induces Fos Gene Transcription and Activates the Transcription Factor Elk-1 in Mature Osteoclasts.” Calcified Tissue International, 76(5), 371–378., doi:10.1007/s00223-004-0099-8. Notes: License Disclosure Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid multiple freeze/thaw cycles. Scientific Category: Kinase Product Type: Cell Line Data shown is lot-specific. Contact us for specific information on other lots. Qty: Rent This Cell Line Quality Assurance Product Datasheets 79380.pdf
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