Human PADI-4, also known as Peptidyl arginine deiminase, type IV, (GenBank Accession No. NM_012387), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q9UM07

PADI-4, Peptidyl arginine deiminase, type IV, Pad

≥80 pmole/min/µg

Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl₂, and 2 mM DTT) containing PADI4 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813–23.
2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384–93.
3. Wang Y, et al. (2004). Science 306 (5694): 279–83.

Avoid freeze/thaw cycles.

Arginine Deiminase

Recombinant Protein