Human PADI-1, also known as Peptidyl arginine deiminase, type I, (GenBank Accession No. NM_013358), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q9ULC6

PADI-1, Peptidyl arginine deiminase, type I, Pad

≥35 pmol/min/μg

Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50 mM NaCl, 10 mM CaCl2, and 2 mM DTT) containing PADI1 and 10 mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation.

aqueous buffer solution

>6 months at –70°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813–23.
2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384–93.
3. Guerrin M, et al. (2003). Biochem. J. 370 (Pt 1): 167–74.

Avoid freeze/thaw cycles

Arginine Deiminase

Recombinant Protein