Novobiocin is an aminocoumarin antibiotic that is produced by Streptomyces niveus. Along with other aminocoumarin antibiotics, novobiocin is a very potent inhibitor of bacterial DNA gyrase. It works by targeting the GyrB subunit of the enzyme involved in energy transduction. Novo-TRX is Texas Red-labeled novobiocin, which can be used as a fluorescence probe in assays for screening DNA gyrase inhibitors.

≥93.3% by HPLC

10 µM solution in anhydrous DMSO

Novo-TRX has been shown to inhibit bacterial DNA gyrase activity with an IC50 of 0.6 =uM.

A red aqueous solution

Store at or below –20°C and avoid multiple freeze-thaw cycles to prevent decomposition.

1. Lamour V, et al., J Biol Chem. 2002 May 24;277(21):18947-53.
2. Glaser BT, et al., J Biomol Screen. 2011 Feb;16(2):230-8.

Avoid freeze/thaw cycles.

Miscellaneous

Inhibitor